WebAvidin is conjugated with FITC under optimal conditions. Concentration 0.5 mg/ml Storage & Handling This solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application FC - Quality tested ICFC - Verified Recommended Usage WebDec 31, 2016 · Preservation and staining of biocytin-filled neurons -Following current-clamp recordings, we 572 followed procedures previously described to remove the recording electrode from the cell and 573 ...
Biotin-FITC monoclonal antibody Immunohistochemistry F4024 - Sigma-Aldrich
WebCF® Dye biocytin is a cell-impermeant, fixable polar tracer that is a conjugate of CF® Dye and biotin with an aldehyde-fixable primary amine. ... Storage conditions can be found on the product information sheet or … WebNEUROBIOTIN-Plus Tracer is a biotin label that is impervious to cleavage and breakdown by biotinidase. It is used for visualizing neural architecture and for the identification of gap junction coupling Features: Fixable primary amine Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells iphone outlook on the web
Immunostaining of Biocytin-filled and Processed Sections
WebSep 30, 2015 · TMR biocytin was readily taken up by cranial nerve roots (Fig. 7), and thus shows promise as an effective alternative to carbocyanine dyes such as DiO and DiI often used in developmental neurobiology to label defined cranial and spinal compartments. Finally, the presence of a lysine moiety in TMR biocytin makes it fixable with … WebBiocytin may be injected into the brain by iontophoresis or by pressure injection methods, and localized in tissue sections using avidin-conjugated labels. It is taken up by neurons and rapidly transpo … Recent studies have shown that biocytin may have multiple applications in neuroanatomical studies. WebBiocytin (Biotinoyl-L-Lysine) Cat. # Size MW Abs Em Solvent Storage 3080 100 mg 372.48 < 300 nm none H 2 O - C and desiccated Features and Biological Applications Biocytin is invaluable as a rapidly transporting, sensitive neuronal … iphone outlook notifications