Hoechst 33342 work concentration

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August undefined, 2024

NettetHoechst stains are well-excited by UV light sources, such as xenon- or mercury-arc lamp, a 355 nm UV argon-ion laser, or a 405 nm violet-diode laser and will emit blue fluorescence in the 430 to 500 nm range. Hoechst 33342 and Hoechst 33258 emit maximally at 454 nm, Hoechst 34580 at 438 nm, and Hoechst S769121 at 504 nm. NettetIridium (III) complex-loaded liposomes as a drug delivery system for lung cancer through mitochondrial dysfunction Cancheng Liao,* Danqiao Xu,* Xiaohong Liu, Yuqi Fang, Jun Yi, Xiaofang Li, Bohong Guo Department of Pharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China *These authors contributed equally to this work …

Hoechst 33342 trihydrochloride (bisBenzimide H 33342 trihydrochloride ...

Nettet26. nov. 2012 · 3 Answers. The Hoechst 33342 dye is similar to DAPI in that both are UV-excited, minor groove-binding, and emit signals proportional to total DNA content. Both … Nettetfrom the same concentration of DNA. 4. REAGENT PREPARATION NOTE: Hoechst 33258 is a possible carcinogen and possible mutagen. Wear gloves and a mask, and work under a fume hood. 4.1 Hoechst 33258 stock dye solution (1 mg/mL): Dilute 1 mL Hoechst 33258 (10 mg/mL solution) with 9 mL distilled, 0.45 µm filtered water. Store in … sirt chc

Hoechst 33342 Protocol for Imaging - Thermo Fisher …

NettetHoechst 33342 and 33258 are quenched by bromodeoxyuridine (BrdU), which is commonly used to detect dividing cells. Hoechst 33342 exhibits a 10 fold greater cell … NettetHoechst stains property may allow fluorescence-activated cell and chromosome sorting according to the extent of DNA synthesis which may provide a bridge between biochemical and cytologic analyses of processes related to DNA replication. [ 2] Application A fluorescent DNA stain qualified for use in Mycoplama staining. Components Nettet26. feb. 2024 · Hoechst 33342, cell-permeant DNA binding dye. Products & Services ... Working at AnaSpec Our jobs Contact us Toggle navigation. Support; 0. Select your ... sirt coupon code

Effects of Hoechst 33342 on C2C12 and PC12 cell differentiation

Hoechst 33342 Cell Signaling Technology

Nettet26. jun. 2007 · For Hoechst staining and side population analysis, cells were retrieved from subconfluent culture by trypsinization, resuspended at 10 6 cells/ml in prewarmed DMEM containing 2% FBS, 10 mM Hepes, then incubated for 90 min at 37 °C with Hoechst 33342 (5 μg/ml final concentration, Sigma) with intermittent mixing. NettetThe optimal cell density, concentration of Hoechst 34580, and stain time ... Note: Both BD Pharmingen™ Hoechst 34580 and BD Pharmingen™ Hoechst 33342 Solution (Cat. No. 561908) may be used interchangeably for most applications according to the Recommended Assay Procedure for each dye. pcl12blxNettetFor standard fluorescence based assays, Hoechst 33342 stock solutions should be diluted in phosphate-buffered saline or mounting medium to a final working concentration of 1 μg/ml. Storage The lyophilized … sirteme

"NettetbisBenzimide H 33342 trihydrochloride can be used to study biochemicals and reagents, fluorescent probes, labels, particles and stains, luminescent compounds and detection … " - Hoechst 33342 work concentration

Hoechst 33342 work concentration

Hoechst or DAPI for nuclear staining? - Biology Stack Exchange

Nettet9. mai 2024 · AMPs may work alone or in combination with antibiotics to diminish drug ... penicillin, streptomycin, propidium iodide, and Hoechst 33342 were purchased from Sigma-Aldrich (St. Louis, MO, USA). JC-1 ... Then, the PI and Hoechst 33342 were added at a final concentration of 1 μg/mL for 20 min. PC 9 cells were observed using the ... NettetSince this counting method did not work for the cell types we were interested in, ... Hoechst 33342, ... A Hoechst concentration of 256 ng/10 4 (1.8 × 10 −6 m) cells significantly increased cell death after 36 h, as confirmed in another study.

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NettetHoechst 33342 will also work with parafomraldehyde or ethanol-fixed cells with modification to the above protocol. The reduction of Hoechst 33342 concentration … NettetHoechst No. 33342 (10 mg/mL in H 2 O stock solution; Invitrogen H1399) Store stock solution at 4°C, protected from light. Phosphate-buffered saline (PBS) Prepare PBS with added CaCl2 and MgCl2 (PBS+). This solution allows cells to adhere to …

NettetWorking concentration Excitation filter (wavelength, nm) Hoechst 33342: 1 mM in distilled water: 1–10 μM: 350: Propidium iodide: 1 mg/ml in distilled water: 1–10 μg/ml: 535: … NettetHoechst 33342 is a cell-permeable fluorescent compound that is able to stain the DNA of eukaryotic and prokaryotic cells by binding with high affinity to the minor groove of AT …

NettetHoechst 33258 Staining Dye Solution (ab228550) is a fluorescent stain for labeling DNA in fluorescence microscopy. This product may be used in fluorescence microscopy, … NettetHoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI[1]. [1]. Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557. ... Working concentration: mg/ml; Method for preparing DMSO master liquid: ...

NettetCharacteristics The blue fluorescent Hoechst 33258, 33342, 34580 and nuclear yellow dyes may be effi- ciently excited with a xenon or mercury-arc lamp or with a UV laser; …

NettetSince this counting method did not work for the cell types we were interested in, ... Hoechst 33342, ... A Hoechst concentration of 256 ng/10 4 (1.8 × 10 −6 m) cells … sirt7 nfatc1Nettet18. nov. 2024 · The absolute Hoechst concentration in the supernatant was calculated by comparison with samples containing known concentrations of Hoechst in Assay Buffer at fixed pH values between 6.0 and 9.0. pcl218 quizletNettetInvitrogen Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used … sirt1 uniprotNettetDescription: IC50 Value: N/A Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. These Bis-benzimides were originally developed by Hoechst AG, … pcl5 covalent compoundNettet4. jun. 2007 · These authors contributed equally to this work. Search for more papers by this author. Nadine Platet, Nadine Platet. INSERM U836, CHU ... then incubated for 90 min at 37 °C with Hoechst 33342 (5 μg/ml final concentration, Sigma) with intermittent mixing. Control cells were given Verapamil (50 μM final concentration, Sigma) to ... pcl4 - lewis structureNettet15. des. 2024 · Hoechst 33342 trihydrochloride is a membrane permeant blue fluorescent DNA stain. Storage: protect from light . For research use only. We do not sell to patients. Hoechst 33342 trihydrochloride Chemical Structure CAS No. : 875756-97-1 Get it April 5 by noon. Order within 19 hrs 48 mins. or Bulk Inquiry * Please select … sirt diet juice recipeNettetHoechst 33342 (2'-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5'-bi-1H-benzimidazole trihydrochloride trihydrate) is a cell-permeable DNA stain that is excited by ultraviolet … pcl-ampho