Phospho- ser/thr pka substrate antibody

Webkinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (2,3). Phospho-PKA substrate-specific antibodies from CST … WebPhospho- (Ser/Thr) PKA Substrate Antibody can be used in high throughput kinase assays and drug discovery applications. It detects proteins and peptides containing a phospho …

PHLPP1 regulates PINK1-parkin signalling and life span

WebBuy pka antibodies from Santa Cruz Biotechnology, Inc. Monoclonal antibodies are available to most protein immunogens. ... PKA Phosphopeptide Substrate Antibody (15B12) sc-65491: mouse IgG 1: N/A: ELISA: NSR: NEW (1) ... Phospho-Specific PKA Antibodies PRODUCT NAME CATALOG # ISOTYPE EPITOPE APPLICATIONS Species Citations WebMar 22, 2024 · Both phosphorylation sites of rpS6 (S235/236 and S240/244) were detected with phospho-specific antibodies. Total protein levels of rpS6, FLAG and β-actin were also detected with immunoblotting. b and c HEK293T and SW480 cells were transfected with FLAG empty vector or FLAG-DRAM1 plasmids for 24 h, and the cells were starved for … inclusion\u0027s k6 https://gatelodgedesign.com

Phospho-(Ser/Thr) PKA Substrate Antibody Cell Signaling …

WebPhospho-(Ser/ Thr) PKA antibody substrates include Apaf-1, CAMKII, Caspase-10, Caspase-2, CREB, and NMDAR1, among others (see Cell Signaling #9621). Alpha--tubulin antibody was purchased from ... WebPhospho- (Ser/Thr) PKA Substrate Antibody. *On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca. WebPhosphoserine/threonine/tyrosine Antibody (61-8300) in ELISA. Direct ELISA analysis of Phosphotyrosine, Phosphoserine, and Phosphothreonine was performed by coating wells of a 96-well plate with 100 µL per well of … inclusion\u0027s k

The regulatory mechanism of Hsp90α secretion and its function ... - PNAS

Category:PKA Substrates SCBT - Santa Cruz Biotechnology

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Phospho- ser/thr pka substrate antibody

PKA Antibody SCBT - Santa Cruz Biotechnology

WebMar 27, 2024 · In healthy myocardium, PLB is phosphorylated at Ser 16 by PKA and at Thr 17 by CaMKII. 38, 39 Phosphorylation of TnI by PKA and PKC occurs at both Ser 23 and Ser 24 and leads to the depression of myofilament Ca 2+ sensitivity. 40-46 We tested whether these phosphorylation events were altered in the diabetic myocardium. WebPhospho-PKA alpha (Ser338) Polyclonal Antibody detects endogenous levels of PKA alpha only when phosphorylated at Ser338. Target Information Protein kinase A (PKA, cAMP …

Phospho- ser/thr pka substrate antibody

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WebOct 19, 2005 · Four hundred microliters NP40-solubilized sperm lysate (2 mg/ml) was incubated with 4 μl of the Phospho-(Ser/Thr) PKA substrate antibody and 40 μl of 50% protein A-Sepharose CL4B beads. Precipitated immunocomplexes, bound to the protein A beads, were washed four times in NP40 lysis buffer without phosphatase inhibitors and … WebA pan-SIK–specific kinase inhibitor reduces both the hyperphosphostate and the elevated sleep need in Sleepy mutant mice, as well as sleep-deprived WT mice. SIK3 has a serine-threonine kinase domain at the N terminus and a protein kinase A (PKA) recognition site S551 in the middle portion ( 7 ).

WebThis antibody preferentially binds peptides and proteins that contain phospho-Ser/Thr preceded by Leu or similar hydrophobic amino acids at the -1 position and followed by Gln at the +1 position. Visit thermofisher.com to view references and figures. WebA good control is the effect of the H89 inhibitor (as well as a known agonist such as dibutyryl cAMP). A possible PKA substrates is CREB (Ser 133) for which there are good phospho-specific antibodies.

WebApr 13, 2024 · In particular, we saw increased phosphorylation of connexin-43 at Ser325/Thr326, and decreased levels of the phospho-peptide, Ser-Thr 320-345 (Figure 4A’; Supplementary Table S4). Notably, the phosphorylation of Ser325 on connexin-43, along with nearby residues Ser328 and Ser330, is mediated by casein kinase 1 and has been shown … WebSee all Phospho - (Ser/Thr) Phe primary antibodies Description Rabbit polyclonal to Phospho - (Ser/Thr) Phe Host species Rabbit Tested applications Suitable for: ELISA, IP, WB, ICC/IF …

WebMonoclonal Antibody for studying PKA Substrate motif [RXX(t/s)] phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-(Ser/Thr) PKA …

WebPolyclonal Antibody for studying PKA (RRXt) substrate motif phosphate. Cited in 210 publications. Validated for WB, IP, IHC, E. Available in 2 sizes. Highly specific and … inclusion\u0027s k5WebFor antiphospho-PKA substrate (RRXS/T) (100G7E) antibody, a rabbit monoclonal antibody against pPKA substrate was used (commercially available from Cell Signaling). For … inclusion\u0027s k7Web2 days ago · Site specific custom made phospho-Ser 932 PHLPP1 antibody was generated by GenScript (Genscript, USA) [12]. Membranes were washed in TBS-T and incubated with horseradish peroxidase-conjugated anti-mouse (Bio-Rad, 170-6516) or anti-rabbit (Bio-Rad, 170-6515) secondary antibodies for 1 h at room temperature. inclusion\u0027s k4WebSee all Phospho - (Ser/Thr) Phe primary antibodies Description Rabbit polyclonal to Phospho - (Ser/Thr) Phe Host species Rabbit Tested applications Suitable for: ELISA, IP, WB, ICC/IF more details Species reactivity Reacts with: Species independent Immunogen Synthetic peptide corresponding to Phospho - (Ser/Thr) Phe . inclusion\u0027s k8WebPhospho-(Ser/Thr) PKA Substrate Antibody detects peptides and proteins containing a phospho-serine/threonine residue with arginine at the -3 position. It is a useful tool in identifying substrates of AGC family kinases, … inclusion\u0027s kbWebIn addition, a phospho- (Ser/Thr) PKA substrate antibody detected bands on immunoblot analysis of trans-sialidase antibody precipitated proteins from parasite lysate and the … inclusion\u0027s k1WebJun 25, 2015 · A phospho-(Ser/Thr) PKA substrate antibody (Cell Signaling Technology) was used to detect in vivo phosphorylation of CP. Constructions of plasmids. inclusion\u0027s ka